Table 2.
Amino acid sequence-altering SNPs in Y68ARev compared to the HSV-1(F) sequence
| Genea | SNPb | Codon changec | Amino acid changed | Suppressor specifice | Frequency in 5 additional Y68A suppressors |
|---|---|---|---|---|---|
| UL8 | C to T (+) | GCG to GTG | A51V | ||
| UL15 | G to A (+) | AGC to AAC | S497N | Y | 0/5 |
| UL15 | G to T (−) | GCC to GTC | A704V | N | |
| UL18 | T to G (−) | ATC to ACC | I160T | Y | 0/5 |
| UL21 | C to T (+) | GCC to GTC | A41V | ||
| UL23 | C to A (−) | GCC to GTC | A272V | ||
| UL26 | G to A (+) | GAA to AAA | E249K | Y | 0/5 |
| UL26/26.5 | G to A (+) | GAT to AAT | D626/319N | Y | 0/5 |
| UL28 | G to A (+) | GTG to ATG | T112 M | N | |
| UL36 | A to G (−) | CTG to CCG | L3099P | N | |
| UL36 | C to T (−) | GCA to ACA | A3082T | N | |
| UL36 | G to A (−) | CGC to TGC | R2646C | Y | 1/5 |
| UL36 | G to A (−) | ACG to ATG | T2584 M | N | |
| UL36 | T to C (−) | ATG to GTG | M2439V | N | |
| UL36 | G to A (−) | TCG to TTG | S2368L | N | |
| UL37 | G to A (−) | CGT to TGT | R651C | N | |
| UL37 | G to A (−) | CGG to TGG | R531W | N | 0/5 |
| UL38 | T to C (+) | TGC to CGC | C35R | ||
| UL39 | C to T (+) | GCT to GTT | A474V | ||
| UL42 | G to A (+) | AGC to AAC | S349N | ||
| UL42 | G to A (+) | AGC to AAC | S358N | ||
| UL48 | T to C (−) | ACG to GCG | T212A | Y | 0/5 |
| UL50 | C to A (+) | CCC to ACC | P134T | ||
| UL51 | G to A (−) | ACG to ATG | T74 M | Y | 0/5 |
| UL52 | C to T (+) | GCT to GTT | A655V | ||
| US8 | C to T (+) | GCG to GTT | A441V | N | |
| US9 | C to T (+) | CGA to TGA | R58Stop | Y | 4/5 |
| US9 | C to T (+) | CTC to TTC | L89F | N | |
| RS1 | A to C (−) | CAG to CCG | Q163P | ||
| RS1 | A to C (−) | GAG to GCG | E266A | ||
| RS1 | G to A (−) | CGC to CAC | R267H | ||
| RS1 | G to A (−) | ACC to GCG | A272T | ||
| RS1 | G to C (−) | GCG to CCG | A273P | ||
| RS1 | A to G (−) | GAC to GGC | D712G |
Genes indicated in boldface have reported functions related to virus assembly, egress, or cell-to-cell spread and were tested to determine whether they were suppressor specific.
All SNPs are listed as nucleotide changes on the top strand of the prototype arrangement of the HSV-1(F) genome. In some cases, this will be the sense strand (+), in other cases, this will be the antisense strand (−), and in the case of RS1, both.
All codons are rendered on the sense strand of the gene.
Amino acids are numbered from the first amino acid encoded by the open reading frame. Amino acid differences indicated in boldface also occur in the published sequence of HSV-1 (17) (GenBank accession NC_001806).
Y, yes; N, no.