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Western blot analysis of SK6 cell lysates infected with E1ΔCys single and double mutant viruses. Detection of E1-E2 heterodimers with anti-E2 MAb WH303 (A) or with an anti-E1 rabbit anti serum (B) and monomeric forms of E1 (C) and E2 (D). SDS-PAGE was performed under nonreducing (A and B) or reducing (C and D) conditions.
