Viral loads and hematologic changes detected in pigs inoculated with double E1ΔCys mutant and parental viruses. (A to C) Virus titers detected in nasal swabs (A), tonsil scrapings (B), and blood (C) after infection with E1ΔCys5/171v, E1ΔCys20/171v, E1ΔCys94/171v, E1ΔCys24/94v, or parental BICv. Each point represents the mean log10 TCID50/ml and standard deviations from at least two animals. Sensitivity of virus detection, ≥log10 1.8 TCID50/ml. (D to F) White blood cell (WBC) (D), lymphocyte (E), and platelet (PLT) (F) counts after infection.