Fig. 4.

Increased generation of Langerhans cells from myeloid progenitor cells latently infected with a viral IL-10 deletion virus. CD34⁺ myeloid progenitor cells were mock infected or latently infected with either parental virus (Parent) or a viral IL-10 deletion virus (vIL-10 del). Cultures of vIL-10 del virus-infected cells supplemented with recombinant viral IL-10 proteins were also generated (vIL-10 del + vIL-10). (A) Flow cytometry scatter plots of cells expressing CD11c and langerin to identify Langerhans cells. (B) Graph depicting the percentage of Langerhans cells. The number of independent biological replicate experiments (n) is shown. Error bars indicate the standard errors of the means. Significant differences (P value of <0.05) between the values for samples were determined by a one-tailed, paired Student's t test and are denoted by an asterisk.