Table 2.
Neutralization sensitivities and Env characteristics of the HIV-1 variants that were isolated from historical and contemporary seroconvertersa
| Virus and period of isolation | Yr of SC | IC50 (μg/ml) |
Length (aa) |
No. of PNGS |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HIVIG | VRC01 | b12 | 2F5 | 447-52D | V1 | V2 | V1V2 | V1 | V2 | V1V2 | ||
| Historical | ||||||||||||
| H19999 | 1985 | 831.3 | 0.121 | 1.12 | >25.00 | >25 | 23 | 39 | 62 | 4 | 2 | 6 |
| H18766 | 1988 | >1,500.0 | 0.094 | >25.00 | 7.29 | >25 | 25 | 38 | 63 | 3 | 2 | 5 |
| H19768 | 1986 | 805.4 | 0.099 | 1.46 | 1.51 | >25 | 25 | 39 | 64 | 3 | 2 | 5 |
| H19342 | 1986 | 846.3 | 0.090 | 1.17 | 2.17 | >25 | 25 | 39 | 64 | 3 | 2 | 5 |
| H19542 | 1985 | 632.9 | 0.524 | 1.41 | 0.69 | >25 | 25 | 42 | 67 | 3 | 3 | 6 |
| Contemporary | ||||||||||||
| P197 | 2005 | 1,009.0 | 0.482 | >25.00 | 0.53 | >25 | 29 | 39 | 68 | 4 | 2 | 6 |
| P180 | 2004 | >1,500.0 | 0.753 | >25.00 | >25.00 | >25 | 27 | 41 | 68 | 5 | 2 | 7 |
| P004 | 2003 | >1,500.0 | 0.345 | >25.00 | 1.76 | >25 | 33 | 39 | 72 | 5 | 1 | 6 |
| P002 | 2003 | 1,316.3 | >5.000 | 5.30 | 0.11 | >25 | 30 | 46 | 76 | 6 | 5 | 11 |
| P127 | 2005 | >1,500.0 | <0.078 | 0.21 | 4.29 | >25 | 38 | 68 | 106 | 4 | 5 | 9 |
| P valueb | 0.042 | 0.283 | 0.204 | 0.883 | 1.000 | 0.008 | 0.230 | 0.088 | 0.006 | 0.380 | 0.043 | |
a
SC, seroconversion; IC50, 50% inhibitory concentration; aa, amino acid; PNGS, potential N-linked glycosylation site(s).
b
Differences in neutralization sensitivity and Env characteristics between the two groups of HIV-1 variants from historical and contemporary seroconverters were evaluated for statistical significance using Student's t test.