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. 2011 Jul;85(14):6832–6843. doi: 10.1128/JVI.00609-11

Fig. 7.

Fig. 7.

Chimeric rIBV-NS110-VN04HA is attenuated both in vitro and in vivo. (A) Multicycle growth curves of the recombinant viruses in MDCK cells infected at an MOI of 0.05 and titrated by plaque assay on MDCK cells. (B) Plaque size phenotypes of the rIBVs in MDCK cells at 3 days p.i. Eight-week-old BALB/c (C) and C57BL/6 PKR−/− (D) mice (n = 6) were infected intranasally with 1 × 105 PFU of the indicated viruses. Average lung titers ± standard deviations are depicted. The limit of detection was 5 PFU. Following viral infection, BALB/c (E) and C57BL/6 PKR−/− (F) mice were weighed daily, and the average body weights of surviving animals in each group up to day 14 p.i. are indicated as percentages of the original body weights.