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. 2011 Jul;85(14):6977–6985. doi: 10.1128/JVI.00179-11

Fig. 6.

Fig. 6.

Subject P2's response to peptide HERV-K FI8 was found to be restricted by the HLA-B51 allele in an ELISPOT-based assay using peptide-pulsed and unpulsed HLA-matched and-mismatched B cells as antigen presenting cells. P2's PBMCs were incubated with the following: lane 1, medium; lane 2, HERV-K FI8 peptide; lane 3, HLA-B51+ B cells pulsed with FI8 peptide; lane 4, HLA-B51+ B cells not pulsed with peptide; lane 5, HLA-B51− B cells pulsed with FI8 peptide; lane 6, HLA-B51− B cells not pulsed with peptide. Lane 7 represents all of the above conditions using B cells alone, without subject P2's PBMCs, in the ELISPOT assay.