Figure 2.

The early flowering phenotype and reduced expression of the vernalization regulator FLC in hos1 mutant plants. (A) Flowering time of C24 wild-type and hos1 mutant plants with and without 2 or 4 wk of vernalization treatment at 0°C. Flowering time is indicated by the total number of rosette leaves at time of flower bud emergence. Shown are the average and standard deviation (n = 15). (B) FLC expression. Total RNA (20 μg per lane) was isolated from wild-type and hos1 mutant seedlings grown in agar plates that were incubated at 0°C for 0, 2, and 4 wk. The tubulin gene was used as a loading control.