Figure 3.
Positional cloning of the HOS1 gene. (A) Physical mapping of HOS1. Genetic mapping delimited HOS1 to the BAC clone T5I7. The hos1 mutation was identified by sequencing and comparing all predicted genes on this BAC from hos1 mutant and wild-type plants. (B) Structure of HOS1 and the position of the hos1 mutation. Positions are relative to the initiation codon. Filled boxes indicate the open reading frame, and lines between boxes indicate introns. The premature stop codon created by the hos1 mutation is boxed.