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. Author manuscript; available in PMC: 2011 Jun 29.
Published in final edited form as: J Cell Biochem. 2010 Sep 1;111(1):94–103. doi: 10.1002/jcb.22666

Fig. 2.

Fig. 2

Confocal microscopy of the subcellular localizations of MsrA-eGFP fusion protein in mouse embryonic stem cells after pEGFP-N1-MsrA fusion expression plasmid transfection for 3 days. A: Stem cells scanned for Mitotracker staining for mitochondria. B: Green fluorescence shows the MsrA-eGFP fusion protein. C: DAPI staining shows nuclei. D: Merged image of A–C showing that MsrA-eGFP localizes in both mitochondria and cytosol, occasionally in nuclei (arrows). E: Phase contrast image of a colony of stem cells. The bar is 10 µm.