Skip to main content
. 2001 Apr 1;15(7):827–832. doi: 10.1101/gad.876201

Figure 2.

Figure 2

The MeCP1 complex contains MBD2 and NuRD. (A) Schematic representation of the steps used to purify the MeCP1 complex. The range of salt concentrations where the MeCP1 complex eluted out of the columns is indicated. (B) Western blot analysis of the fractions derived from DEAE-5PW column. (C) Gel mobility shift assay of the fractions derived from the last purification step. Methylation-dependent shift and free probes are indicated. (D) A silver-stained gel showing that a group of 10 polypeptides copurify with the methyl-CpG-binding activity. The identities of these polypeptides are confirmed by Western blot analysis, shown in panel E.