Figure 3.

MBD2 and NuRD exist in the same protein complex. (A) A silver-stained gel showing that the same 10 polypeptides are immunoprecipitated by both MTA2 and MBD2 antibodies. MBD2 has characteristically faint staining with silver (Figs. 2D,3A), but stains better with Coomassie (Fig. 3C). The identities of the proteins and size markers are indicated. (B) Western blot analysis of the same samples used for silver staining in panel A. Antibodies used for immunoprecipitation and Western blotting are indicated on the top and left of the panel, respectively. (C) Coomassie staining of the MeCP1 complex. (D) Mi2 enhances MBD2-mediated transcription repression. The amount of reporter and effector plasmids used in each transfection is indicated. Transfection efficiencies were normalized using β-galactosidase assays. The data shown represent the average of two independent experiments. Variations between experiments are depicted by the error bars.