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. 2011 Jul;338(1):114–124. doi: 10.1124/jpet.111.180257

Fig. 3.

Fig. 3.

A, progress curves for inhibition of human FAAH by PF-04457845 in the presence (5–625 nM) or absence of PF-04457845. B and C, FAAH inhibition by PF-04457845 is time-dependent. Initial rates were determined using a microplate reader as described under Materials and Methods. The concentrations of PF-04457845 were varied from 1 nM to 10 μM. Three separate experiments were performed, and the averages ± S.D. were plotted as percentage of control versus inhibitor concentration and fit to the equation, y = 100/ [1 + (x/IC50)z], using KaleidaGraph (Synergy Software, Reading, PA), where IC50 is the inhibitor concentration at 50% inhibition and z is the Hill slope (the slope of the curve at its inflection point). The IC50 curves are shown for inhibition of human FAAH (B) and rat FAAH (C) with preincubation times of 1, 5, 30, and 60 min.