Figure 3.
Expression of the QKI isoforms. (A) Untransfected (control) or myc–QKI-5, myc–QKI-6, myc–QKI-7, and myc–QKI-G were expressed in HeLa cells. The cell lysates were separated by SDS-PAGE and analyzed by immunoblotting with anti-myc (lanes 1–5) or anti-pan QKI antibodies (lanes 6–10). The molecular mass markers are shown at left in kD. (B) HeLa cells were transfected with the indicated myc–QKI as in A and analyzed by immunoblotting with anti-QKI-5 (lanes 1–4), anti-QKI-6 (lanes 5–8), and anti-QKI-7 (lanes 9–12) antibodies. The migration of QKI-5, QKI-6, and QKI-7 is indicated. (C) NIH 3T3 cells were transfected with expression plasmids expressing the indicated GFP fusion protein. The cells were lysed and analyzed by immunoblotting with anti-pan QKI antibodies.