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Ab/SOD conjugate specifically protects the endothelial barrier from VEGF insult. Ab/SOD, but not Ab/catalase, inhibited VEGF-induced endothelial permeability. HUVEC monolayers were preincubated with conjugates of Ab/catalase and Ab/SOD (100 μg/ml, 30 min) or DPI (10 μM, 1 h). Cells then were stimulated with VEGF (200 ng/ml) for 30 min followed by the FITC-dextran influx assay. Data shown are means ± S.D. (n = 4; *, p < 0.05; **, p < 0.01).
