TABLE 3.
Enzyme | Decay Rate Constant at 52°C | 52°C t50 Ratio+ | Decay Rate Constant in Urea | Urea t50 Ratio+ |
---|---|---|---|---|
min−1 | min−1 | |||
wtT547 | 0.011 ± 0.002 | 1.0 | 0.040 ± 0.003 | 1.0 |
R3Q | 0.024 ± 0.008 | 0.36* | 0.049 ± 0.005 | 0.82* |
H322N | 0.022 ± 0.004 | 0.48** | 0.060 ± 0.01 | 0.63* |
D134H | 0.30 ± 0.07 | 0.036*** | 0.25 ± 0.04 | 0.17*** |
D134H/R136Q | 0.070 ± 0.02 | 0.18**†† | 0.096 ± 0.006 | 0.42***††† |
t50 mutant/t50 wild type.
P > 0.05 (vs. wtT547).
P = 0.05 (vs. wtT547).
P < 0.05 (vs. wtT547).
P = 0.05 (vs. D134H).
P < 0.05 (vs. D134H).