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. Author manuscript; available in PMC: 2011 Jun 29.
Published in final edited form as: Nat Methods. 2008 Sep 21;5(10):877–879. doi: 10.1038/nmeth.1253

Figure 1.

Figure 1

Simultaneous detection of a unique sequence and a repeated sequence in individual mRNA molecules. a) Schematic depiction of the construct used. The 48 probes used to detect the GFP coding sequence were labeled with Alexa 594 and the four different probes used to detect the tandem repeat in the 3′ UTR were labeled with TMR. b) Maximum intensity merges of a pair of z-stacks of fluorescent images of CHO cells taken in the Alexa 594 channel (left) and the TMR channel (right) corresponding to GFP coding region probes and UTR probes, respectively. c) False color merge of the images in b) enclosed by the squares, with circles representing computationally identified mRNA particles. All scale bars are 5 μm long.