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. 2001 Apr 1;29(7):1524–1533. doi: 10.1093/nar/29.7.1524

Figure 5.

Figure 5

Arabidopsis ADA2a, ADA2b and GCN5 are transcribed in roots and leaves. Northern filters were prepared with total RNA (20 µg) isolated from roots (R) or leaves (L) of plants grown in vitro at 22°C or from leaves of cold acclimated plants grown in soil (Lc). Filters were hybridized with cDNA probes for either ADA2a, ADA2b or GCN5 and then stripped and hybridized in succession with cDNA probes for eIF-4A and COR15a. Autoradiography for the ADA2a, ADA2b, GCN5 and eIF-4A probes was for 22 h using a single intensifying screen at –80°C; exposure for the COR15a probe was for 4 h at room temperature.