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. 2001 Apr 15;15(8):995–1006. doi: 10.1101/gad.873501

Figure 3.

Figure 3

Circadian accumulation of e4bp4, dbp, hlf, and tef mRNAs in the mouse liver. (A) Northern blot analysis of the total RNAs (10 μg) isolated from the liver of DD-housed mice at different time points. The sizes of e4bp4 and hlf mRNAs are shown on the right. No significant variation in the amounts of loaded mRNA was detected by hybridization with a glyceraldehyde-3-phosphate dehydrogenase (g3pdh) cDNA probe. (B) Rhythmic expression of e4bp4 (black) is plotted in comparison with dbp (red), hlf (blue), and tef (green). The hlf mRNA levels represent the sum of the quantitated radioactivities of 4.5-kb and 2.6-kb transcripts. Normalized mRNA levels are shown with each peak value adjusted to 1.0. (C) Western blot analysis of liver nuclear extracts prepared from DD-housed mice, with anti-E4BP4 antiserum (upper panel) and anti-DBP antiserum (lower panel). (D) Rhythmic expression of E4BP4 (black) is plotted in comparison with DBP (red). Protein levels were determined by Western blot analysis in (C). The peak values are adjusted to 1.0.