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. 2001 Apr 15;15(8):995–1006. doi: 10.1101/gad.873501

Figure 4.

Figure 4

Transcriptional regulation by E4BP4 and PAR proteins. (A) Transcriptional regulation of the mPer1 promoter by E4BP4 and each PAR protein alone. A reporter plasmid (10 ng) containing a 1.3-kbp mPer1 promoter including the DBP-binding site, and increasing doses of each expression plasmid, were cotransfected. Each value is the mean ± SEM of triplicate results for a single assay. A similar pattern of activation or suppression was reproduced in another experiment. (B) Transcriptional regulation of the mPer1 promoter by the coexpression of E4BP4 and each PAR protein. Presence (+) or absence (−) of DBP-, HLF-, TEF-, and E4BP4-expression plasmids (1000, 500, 25, and 1000 ng, respectively) is noted. (C) Transcriptional regulation of an HSV-TK-driven reporter plasmid containing a DBP-binding site derived from the mPer1 promoter. The presence (+) or absence (−) of DBP-, HLF-, TEF-, and E4BP4-expression plasmids (500, 100, 5, and 500 ng, respectively) is noted. The total amount (1 μg) of expression plasmids per well was adjusted by adding a pcDNA3 plasmid. (B,C) Each value is the mean ± SEM of three replicates for a single assay. The results shown are representative of at least three independent experiments.