. 2011 May 31;9:37. doi: 10.1186/1741-7007-9-37

Table 1.

Quantification of ¹³C enrichments in relevant metabolites by ¹H total correlation spectroscopy (TOCSY) at 48 h

Compound	Percentage ¹³C
Uracil in UXP

¹²C6¹²C5	52 ± 3

¹²C6¹³C5	14 ± 2

¹³C6¹²C5	16 ± 2

¹³C6¹³C5	18 ± 2

Asp

¹²C2¹²C3	46 ± 3

¹²C2¹³C3	15 ± 2

¹³C2¹²C3	19 ± 2

¹³C2¹³C3	21 ± 2

AXP ribose

¹²C1'¹²C2'	8 ± 2

¹³C1'¹³C2'	92 ± 2

UXP ribose

¹²C1'¹²C2'	4 ± 1

¹³C1'¹³C2'	96 ± 2

UDP GlcNAc

Glc ¹²C1¹²C2	9 ± 2

Glc ¹³C1¹³C2	91 ± 2

Lactate

¹²C2¹²C3	19 ± 2

¹³C2¹³C3	81 ± 2

Alanine

¹²C2¹²C3	23 ± 2

¹³C2¹³C3	77 ± 2

Glutamate

¹²C2¹²C4	63 ± 2

¹²C2¹³C4	32 ± 2

¹³C2¹²C4	< 1

¹³C2¹³C4	5 ± 2

¹²C2¹²C3	81 ± 2

¹²C2¹³C3	6 ± 2

¹³C2¹²C3	5 ± 2

¹³C2¹³C3	8 ± 2

Atom percentage ¹³C enrichments in metabolites after 48 h incubation in [U-¹³C]-glucose were determined from two-dimensional ¹H TOCSY experiments as described in the Methods section. Uracil in UXP refers to the uracil ring in the free nucleotide pool comprising UTP, UDP and UMP. AXP and UXP ribose refer to the ribose moiety in the free nucleotide pools. UDP-N-acetyl-D-glucosamine (UDP-GlcNAc) refers to the C1-C2 of the glucose moiety of the free UDP-GlcNAc pool.