Table 2.
Strains used in this study
| Strain
|
Genotype
|
Sourcea
|
|---|---|---|
| W303-1b | MATα ade2-1 can1-100 his3-11 leu2-3, 112 trpl-1 ura3-1 | R. Rothstein |
| MC89 | W303 MATα SUM1-1 | D. Shore |
| TD4 | MATacan1 his4-519 leu2-3,112 trpl ura3-52 | G. Fink |
| JRY2726 | MATahis4 | P. Schatz |
| JRY4565 | W303 MATα sir2Δ | |
| JRY7145 | W303 MATα hst1Δ::KanMX | |
| JRY7146 | W303 MATα orc5-1 | |
| JRY7147 | W303 MATα orc2-1 | |
| JRY7148 | W303 MATα SUM1-1 hst1Δ::KanMX | |
| JRY7149 | W303 MATα SUM1-1 orc5-1 | |
| JRY7150 | W303 MATα SUM1-1 orc2-1 | |
| JRY7151 | W303 MATα sir2Δ::TRP1 hstlΔ::KanMX sumlΔ::LEU2 | |
| JRY7152 | W303 MATα sir2Δ::TRP1 SUM1-1 | |
| JRY7153 | W303 MATα sir2Δ::HIS3 7myc-SUM1-1 | |
| JRY7154 | W303 MATα sir2Δ::TRP1 SUM1-1 hstlΔ::KanMX | |
| JRY7156 | W303 MATα sir2Δ::TRP1 hst1Δ::KanMX | |
| JRY7157 | W303 MATα sir2Δ::TRP1 SUM1-1 orc5-1 | |
| JRY7158 | W303 MATα sir2Δ::HIS3 7myc-SUM1-1 orc5-1 | |
| JRY7159 | W303 MATα sirΔ::TRP1 SUM1-1 orc2-1 | |
| JRY7160 | W303 MATα sir3Δ::LEU2 | |
| JRY7161 | W303 MATα sir3Δ::LEU2 SUM1-1 | |
| JRY7162 | W303 MATα sir3Δ::LEU2 SUM1-1 hst1Δ::KanMX | |
| JRY7163 | W303 MATα sir3Δ::LEU2 hstlΔ::KanMX | |
| JRY7164 | W303 MATα sir3Δ::LEU2 SUM1-1 orc5-1 | |
| JRY7165 | W303 MATα sir4Δ::HIS3 | |
| JRY7166 | W303 MATα sir4Δ::HIS3 SUM1-1 | |
| JRY7167 | W303 MATα sir4Δ::HIS3 SUM1-1 hstlΔ::KanMX | |
| JRY7168 | W303 MATα sir4Δ::HIS3 hstlΔ::KanMX | |
| JRY7169 | W303 MATα sir4Δ::HIS3 SUM1-1 orc5-1 | |
| JRY7170 | W303 MATα sum1Δ::LEU2 | |
| JRY7172 | W303 MATα sir2Δ::HIS3 3myc-SUM1 | |
| JRY7173 | W303 MATα sirlΔ::LEU2 SUM1-1 | |
| JRY7174 | W303 MATα sirlΔ::LEU2 sir2Δ::TRP1 SUM1-1 | |
| JRY7176 | W303 MATα sir2Δ::HIS3 7myc-SUM1-1 orclΔ::TRP1 LEU2::orclΔl-235 | |
| JRY7177 | W303 MATα sir2Δ::HIS3 7myc-SUM1-1 orclΔ::TRP1 LEU2:ORC1(Q236H,I237M) | |
| JRY7178 | W303 MATα sir2Δ::HIS3 7myc-SUM1-1 hstlΔ::KanMX |
a
Unless otherwise indicated, all strains were produced for this study.