Table 3.
Comparison of mean (standard deviation) Ct of reference genes analyzed by RT-qPCR, for the 4 centres
| Reference Gene | Centre | All centres | |||
|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | ||
| TFR | 23.85 (0.87) | 24.09 (1.28) | 24.23 (1.01) | 26.30 (1.82) | 24.34 (1.47) |
| β-actin | 17.15 (0.58) | 17.34 (1.19) | 17.10 (0.62) | 18.32 (0.88) | 17.38 (0.94) |
| TBP | 26.46 (0.53) | 26.53 (1.18) | 26.81 (0.47) | 28.11 (1.10) | 26.77 (1.03) |
| GAPDH | 19.78 (0.94) | 19.99 (1.27) | 19.90 (0.77) | 20.47 (1.05) | 19.96 (1.07) |
| RPLPO | 19.44 (0.60) | 19.43 (1.06) | 19.25 (0.78) | 20.51 (0.93) | 19.59 (0.91) |
| GUS | 22.84 (0.71) | 22.98 (1.23) | 23.05 (0.76) | 24.83 (1.33) | 23.21 (1.21) |
| p-value referring to ANOVA test for each reference gene, when excluding corresponding centre | |||||
| TFR | < 0.001 | < 0.001 | < 0.001 | 0.144 | < 0.001 |
| β-actin | < 0.001 | < 0.001 | < 0.001 | 0.256 | < 0.001 |
| TBP | < 0.001 | < 0.001 | < 0.001 | 0.165 | < 0.001 |
| GAPDH | 0.075 | < 0.001 | 0.003 | 0.416 | 0.006 |
| RPLPO | < 0.001 | < 0.001 | < 0.001 | 0.562 | < 0.001 |
| GUS | < 0.001 | < 0.001 | < 0.001 | 0.457 | < 0.001 |