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Determination of rRNA methylation sites predicted by the novel snoRNAs. Lane 1, control reaction at 1.5 mM dNTP; lane 2, primer extension at 4 µM dNTP; lanes A, C, G and T, the rDNA sequence ladder. The sites of ribose methylation were revealed by RT pauses at low dNTP concentrations. Arrows indicate potential methylation sites predicted by the novel snoRNAs.
