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Detection of a polycistronic snoRNA transcript by RT–PCR with primer pair Z37aF/Z42at. Lane DNA, control PCR performed on the total DNA; lane RT–PCR, PCR amplification after reverse transcription of total RNA; lane PCR-C1, control PCR performed on total RNA without reverse transcription; lane PCR-Co, control PCR carried out with water as template; lane M, molecular weight marker (pBR322 digested with HaeIII).
