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. 2011 Jun 29;6(6):e21501. doi: 10.1371/journal.pone.0021501

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Laurson, Raj.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immunofluorescence of HPV16E7 protein in sub-confluent NIKS+E7 treated with different compounds to arrest the cell cycle at different stages. (a) Confluent control (b) untreated sub-confluent control and sub-confluent (c) mimosine (G1 block), (d) thymidine (S-phase block), (e) etoposide (G2 block) and (f) nocodazole (mitosis block). Cell cycle blocks were confirmed in parallel by propidium iodide staining analysed by flow cytometry.