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. 2011 Jun 29;6(6):e21462. doi: 10.1371/journal.pone.0021462

Figure 4. Activation of ERK1/2 and IκBα by RANKL and M-CSF in mature OCs.

Figure 4

Mature OCs were serum starved for 18 h then acutely treated with RANKL (125 ng/mL) or M-CSF (25 ng/mL) alone, or in combination. Cytoplasmic protein extracts were harvested and subjected to SDS-PAGE followed by western analysis utilising antibodies against total and phosphorylated ERK1/2 and IκBα. A. Augmentation of phospho-ERK1/2 in the presence of RANKL and M-CSF. B. M-CSF has no effect on phospho-IκBα; representative data from 2 independent experiments.