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Rev-GFP fusion protein or Rbfox3 variant 3 with an N-terminal flag-tag were expressed in N2A cells and (A) left untreated or (B) treated with 10 ng/ml leptomycin B for 4 hrs prior to fixation and staining with anti-NeuN and DAPI, or just DAPI in the case of Rev-GFP. Leptomycin B treatment caused Rev-GFP to be retained in the nucleus, but caused no change in the sub-cellular localization of Fox3v3.
