Table 3. Influenza virus RNA and host gene mRNA levels in tracheal secretions 24 hours after initial A/Memphis/7/2001 (H1N1) challenge; H1N1 re-challenge; or challenge with heat-inactivated H1N1.
| mRNA expression fold change relative to pre exposure | |||||
| Animal Number | Peak log10 vRNA copies/ml | IFN-α | OAS | MxA | IL-6 |
| No Treatment prior to H1N1 re-challenge | |||||
| 30924 | 3.7 | −5.1 | 4.3 | 3.2 | 10.9 |
| 30933 | 3.9 | NAa | 27.6 | 11.0 | 3.4 |
| 33470 | 4.6 | 162.3 | 32.3 | 15.5 | 13.6 |
| Mean | 4.1 | 78.6 | 21.4 | 9.9 | 9.3 |
| Anti-CD8 Treatment prior to H1N1 re-challenge | |||||
| 30811 | 4.8 | NA | NA | NA | 86.9 |
| 30831 | 3.1 | 3.4 | 5.1 | 3.5 | 22.7 |
| 30851 | 2.2 | 2.5 | 3.9 | 5.0 | 12.6 |
| Mean | 3.4 | 2.9 | 4.5 | 4.3 | 40.7 |
| Anti-CD20 treatment prior to H1N1 re-challenge | |||||
| 30616 | 2.5 | −2.7 | 2.5 | 1.5 | 22.1 |
| 30921 | 4.2 | NA | NA | NA | NA |
| 35125 | 4.3 | −34.6 | 5.7 | 16.4 | 6.0 |
| Mean | 3.7 | −18.6 | 4.1 | 8.9 | 14.0 |
| Initial H1N1 challenge b | |||||
| 33073 | 5.8 | 3910.6 | 53.7 | 29.4 | 287.8 |
| 33178 | 5.4 | 11340.0 | 101.8 | 15.3 | 72.5 |
| 34421 | 6.4 | 1606.1 | 65.4 | 19.2 | 5786.6 |
| Mean | 5.9 | 5618.9 | 73.6 | 21.3 | 2048.9 |
| Initial challenge with Heat-inactivated H1N1 b | |||||
| 31392 | 2.9 | −11.3 | 1.7 | 5.3 | −2.8 |
| 31625 | 3.5 | 40.6 | 2.0 | 5.5 | NA |
| 31705 | 2.4 | 1.1 | 1.8 | 2.6 | −4.3 |
| 31864 | 3.1 | −14.0 | −1.9 | 10.8 | −2.5 |
| Mean | 3.0 | 4.1 | 0.9 | 6.0 | −3.2 |
a
= target gene mRNA levels could not be determined due to poor amplification of GAPDH mRNA.
b
= data adapted from Carroll et al, J. Immunol. 2008 [12].