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. 2001 May 1;15(9):1093–1103. doi: 10.1101/gad.874201

Figure 4.

Figure 4

Ultraviolet-induced crosslinking of [α-32P] GTP to CodY. (A) CodY (3 μM), overexpressed and purified from Escherichia coli BL21/λDE3, was incubated with 0.1 mM [α-32P] GTP and 0.1, 0.5, 1, 2, and 10 mM unlabelled GTP, ATP, CTP, or UTP and irradiated with UV light. Irradiated samples were analyzed by SDS-PAGE (12% polyacrylamide) and exposed to a phosphorimager screen. (B) Quantitation of results in part (A). Unlabelled GTP, closed squares; unlabelled ATP, closed diamonds; unlabelled CTP, closed circles; unlabelled UTP, closed triangles.