Skip to main content
. 2011 Jun 29;6(6):e21738. doi: 10.1371/journal.pone.0021738

Figure 5. Neutralization of Mtb VapC toxicity in M. smegmatis by cognate antitoxin expression.

Figure 5

A: Cognate toxin-antitoxin modules were co-expressed as an operon under control of the Tet-regulated promoter. Serial dilutions were plated on 7H10 agar alone (-ATc) or with ATc at 25 ng/ml (+ATc). B: Toxin and antitoxin genes were expressed separately on Tweety (vapC) or L5-based integration vectors (vapB) under control of Tet- or acetamide-regulated promoters, respectively. Serial dilutions were plated on 7H10 agar alone or supplemented with Gm and Km, or Gm and ATc (50 ng/ml).