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. 2011 Jun 29;6(6):e21738. doi: 10.1371/journal.pone.0021738

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Ahidjo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Interactions between VapCs and either cognate or non-cognate VapBs were tested by co-transformation and scoring for growth on selection (LT) vs. media of differing stringency. Three independent colonies of each strain were re-suspended in sterile water, the cell density adjusted to an OD₆₀₀ of 1 and aliquots spotted on plates. LT, Leu-Trp; LTH, Leu-Trp-His; LTHA, Leu-Trp-His-Ade dropout-supplemented media.