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. 2011 Jun 29;6(6):e21738. doi: 10.1371/journal.pone.0021738

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Ahidjo et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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VapC proteins Rv0065 and Rv0617 display sequence-selective RNase activity against an RNA substrate of ∼150 bases in the presence of 6 mM MgCl₂. Activity is Mg²⁺-dependent as addition of 12 mM EDTA abolishes activity (lane 4). Ribonuclease activity is also inhibited in the presence of VapB (lane 9). Addition of VapC (+VapC) results in degradation of the RNA substrate over a period of 5 – 60 minutes (lanes 5–8). RNA only controls (-VapC) show no contaminating ribonuclease activity (lanes 2 & 3). The molecular weight marker shows molecular masses of single-stranded RNA in number of bases (lane 1).