Skip to main content
. 2001 May 1;15(9):1104–1114. doi: 10.1101/gad.879301

Figure 5.

Figure 5

Glucose-regulated localization of Gal83–GFP in mutant strains. (A) The subcellular localization of Gal83–GFP expressed from the 2-μm plasmid pRT14 was monitored in strains with the indicated genotypes (MCY4458, MCY2634, MCY2916, and MCY2693). Cells were grown to mid-log phase on synthetic medium with 2% glucose (Glu) and shifted for 20 min to 5% glycerol (Gly). (B) Gal83–GFP was expressed from pRT12 in strain MCY3541 (hxk2Δ) and from pRT14 in MCY4408 (reg1Δ). Cells were grown in 2% glucose and shifted for 20 min to 2% glycerol. (GFP) green fluorescent protein.