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. Author manuscript; available in PMC: 2012 Jun 1.
Published in final edited form as: J Orthop Res. 2010 Dec 23;29(6):828–833. doi: 10.1002/jor.21319

Figure 3.

Figure 3

(A) 35 cycles of RT-PRC show P2Y2 mRNA expression level in differentiated ATDC5 cells was about 2.76 fold higher relative to that in undifferentiated cells. (B) Western blotting analysis shows P2Y2 protein level in differentiated ATDC5 cells was about 3 fold higher than that in undifferentiated cells. (C) Increases in ERK1/2 phosphorylation in ATDC5 cells transfected with pcDNA3-P2Y2 (P2Y2) or empty vector pcDNA3 (Vector) were observed at 5 and 15 minutes in response to oscillatory fluid flow. (D) Bar graph representation of ERK1/2 phosphorylation quantified by scanning densitometry normalized to GAPDH. (*, p<0.05) Each bar represents the mean ± S.E. and each experiment was repeated on 3–5 times.