Figure 2.

Activation of ribozyme function by two effectors. Plots of the natural logarithm of the fraction of TF1 that remains uncleaved versus time. (A) Both FMN (encircled F, 1 mM final concentration) and theophylline (encircled T, 1 mM final concentration) were added simultaneously to a reaction mixture at t = 120 min. Similarly, effectors were added independently in (B) and (C) at t = 60 min and t = 120 min, as indicated. The fraction of precursor RNAs that self-cleaved were established as described in Materials and Methods.