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. 2011 Apr 13;39(8):2114–2129. doi: 10.1007/s10439-011-0307-4

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© The Author(s) 2011

This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Tartrate-resistant acid phosphatase (TRAP) staining of bone marrow mononuclear cells pre-cultured for 3 days on pure PLGA and PLGA/S-BG composites in the presence of MCS-F, followed by 3-week culture in standard medium on tissue culture plastic. Cells pre-cultured on pure PLGA (a) and PLGA/S2-21 composites (b) displayed typical fibroblastic morphology and they were negative for TRAP. Positive TRAP staining and osteoclast-like morphology of cells was observed for cells pre-cultured on PLGA/A2-21 composites (c, d)