Figure 2.

Indirect immunofluorescence of a strain expressing HMGB1-1xFLAG from the chloroplast genome. Wildtype (137c) or transgenic algae expressing aptA::HMGB1-1xflag were fixed on to poly-L-lysine treated coverslips, stained with mouse anti-FLAG primary antibody, washed and then stained with anti-mouse-texas red (TR). The DNA was stained with DAPI. The merge image demonstrates that HMGB1 does not co-localize with the nucleus, as indicated by the large DAPI-staining body, as would be expected from a chloroplast-localized protein.