Figure 1.

Structure of the TSC1 locus and predicted structure of the TSC proteins. (A) Genomic structure of the TSC1 locus. The TSC1 gene is located between CG6129 and sec10 on 3R. The exons are indicated with thick lines and the introns are shown as thin lines connecting adjacent exons. The transcription start site is indicated with a single line with an arrow at the end. The translation start site is indicated with double lines with an arrow at the end. The genomic DNA fragment used in the rescue construct is indicated. (B) Schematic structures of the Drosophila TSC1 and TSC2 proteins. TSC1 is a predicted 1100 aa polypeptide containing two coiled-coil domains (CC1 and CC2). The molecular lesions in TSC1¹² and TSC1²⁹ are indicated. TSC2 is a predicted 1847 aa polypeptide containing a GAP domain.