Figure 3.

TSC1 controls cellular growth and proliferation during imaginal disc development. In all panels, TSC1 mutant clones were generated by FRT/FLP and marked by the absence of Ubi-GFP signal (green). (A–C) Confocal images of a third-instar eye disc containing a large TSC1⁻ clone (arrow). The adjacent area of brighter green staining represents a +/ + twin spot (arrowhead). The disc was stained for the neuronal specific nuclear Elav protein (red). Three images are shown, one of GFP (A), one of Elav staining (B), and one of superimposed GFP and Elav staining (C). Note the increased size of the mutant cell nuclei and the dramatically increased area of the mutant clone as compared with its twin spot. (D–F) Confocal images of a portion of a third-instar eye disc containing TSC1 mutant clones (arrows). The disc was stained with phalloidin (red), which highlights the outlines of the cells. Three images are shown, one of GFP (D), one of phalloidin staining (E), and one of superimposed GFP and phalloidin staining (F). Note the increased size of the mutant cells. (G–H) Confocal images of a portion of a third-instar wing disc containing a TSC1 mutant clone (arrow). The disc was stained with fibrillarin (red), which labels the nucleolus of the cells. Three images are shown, one of GFP (G), one of fibrillarin staining (H), and one of superimposed GFP and fibrillarin staining (H). Note the increased nucleolar size of the TSC1^– cells.