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Immunoblotting for p-Akt(S473) and ER antibodies. MCF-7 sub-lines were treated with the indicated concentrations of BEZ235 or GSK212 overnight. Actin is shown as a loading control. Bands were normalized to actin and bars represent changes in fold compared with untreated cells and expressed as the mean ± standard deviation from three experiments. Representative blots are shown above bar graph. *Significant difference from treatment control (p < 0.05).
