Table 2.

LC3b turnover is stimulated by starvation and suppressed by refeeding

	LC3b-II [ng per mg total protein]
Treatment:	Fed ad lib.	Starvation	Refed
PBS	37.91 ± 13.93	49.77 ± 25.97	36.20 ± 11.39
Leupeptin	78.18 ± 19.79	109.21 ± 23.57	39.21 ± 9.88
Change After leupeptin (LC3b-II flux)	37.21 ± 10.38*	53.94 ± 14.28*	3.00 ± 2.17#

Quantification LC3b-II content in liver 3 hours after injection with 40 mg/kg leupeptin or PBS. Data is pooled from 2 independent experiments (n = 9 for ad lib. diet; n = 9 for animals calorie-starved for 18 hours; n = 6 for animals refed for 1 hour after 17 hours of starvation). Values represent the mean ±1 SD.

^*p = 0.04;

^#p < 0.001 Student's 2-Tailed t-Test versus mice fed ad lib.