Figure 2.

Atrial natriuretic peptide is increased in both control and E2F1 knockout mice after treatment with either isoproterenol or angiotensin II. Expression of atrial natriuretic peptide (ANP), a molecular marker of hypertrophy, was significantly increased upon treatment with isoproterenol of angiotensin II in both E2F1 +/+ and E2F1 -/- compared to non treated animals. Real time expression of ANP was normalized to expression of GAPDH, a housekeeping gene, and presented as fold change. No difference was seen between E2F1 +/+ and E2F1 -/-. *p<0.05 versus no treatment