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. 2011 Mar-Apr;2(2):87–91. doi: 10.4161/nucl.2.2.14637

Figure 2.

Figure 2

Depletion of Nup188 promotes targeting of a reporter protein to the INM in human cells. HeLa cells were transfected with either a control siRNA or two different siRNAs to Nup188 (9 nM siRNA, 2 × 48 hrs). After 72 hrs, cells were transiently transfected with 2GFP-SUN2(1-260) (Turgay et al. 2010). Cells were either fixed in 1% PFA and analyzed by confocal microscopy or cell extracts were analyzed by immunoblotting using an antibody against Nup188. Loading control: Ponceau S staining of nitrocellulose membrane used for antibody detection of Nup188.