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. 2001 Jun 1;15(11):1406–1418. doi: 10.1101/gad.889901

Figure 7.

Figure 7

Akt targets hexokinase (HK) activity to the mitochondria. Total hexokinase activity was determined enzymatically in total cell lysates and mitochondrial lysates. HK activities were normalized to the protein content of the sample and the percentage of HK activity localized at the mitochondria was calculated. The graph shows the average (±SE) of three independent experiments. (A) Mitochondrial hexokinase activity in Rat1a/pBP and Rat1a/MyrAkt cells 0 and 2 h after induction of apoptosis (P < 0.002). (B) Western blot analysis showing expression of Akt in Rat1a/pBSTR1MyrAkt cells in the presence or absence of tetracycline (Tet). Arrow indicates endogenous Akt protein in the presence of Tet and inducible MyrAkt protein in the absence of Tet. (C) Amount of total hexokinase activity that is localized at the mitochondria in Rat1a/pBSTR1MyrAkt or in vector control Rat1a/pBP cells grown in the presence or absence of 1 μg/mL Tet (P < 0.001).