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. 2011 Mar-Apr;2(2):136–145. doi: 10.4161/nucl.2.2.15134

Figure 1.

Figure 1

Preparation and specificity of anti-TcPCNA antibodies. Coomassie Blue stained SDS-PAGE of E. coli BL21 total extracts (A) without (−) or after IPTG induction (+) or (B) after Ni-agarose purification. Arrows indicate rTcPCNA migration. (C) T. cruzi epimastigotes were boiled in the SDS-PAGE loading buffer, and the supernatants were analyzed by SDS-PAGE, followed by western blot using either anti-rTcPCNA immune or pre-immune serum rabbit. The numbers on the left of each gel correspond to the molecular weight standards.