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. 2001 Apr 1;29(7):1582–1589. doi: 10.1093/nar/29.7.1582

Figure 5.

Figure 5

Figure 5

Expression of the mitochondrially targeted EcoRI endonuclease is controlled by galactose induction. (A) Transcription of the CDC9–EcoRI plasmid is tightly controlled by the GAL1 promoter. (Left) Northern blot analysis of untransformed strain 2780-49BWT yeast and strain 2780-49BWT yeast harboring the CDC9–EcoRI construct before and after galactose induction. (Right) Ethidium bromide stained agarose gel of the RNA used in the northern blot. (B) A CDC9–GFP fusion protein is targeted to yeast mitochondria and detected by confocal fluorescence microscopy. (Top left) Yeast expressing the CDC9–GFP fusion; (top middle) yeast stained with the mitochondria-specific dye MitoTracker Red; (top right) yeast expressing the CDC9–GFP fusion protein stained with MitoTracker Red. The bottom set of panels depict GFP green and red fluorescence and the MitoTracker Red red and green fluorescence.