SU(VAR)2-10 is generally associated with squashed polytene chromosomes and does not colocalize with HP1 at telomeres. Indirect immunofluorescence showing SU(VAR)2-10 (green) on squashed polytene chromosomes (DAPI-stained DNA is blue in all panels). (A) SU(VAR)2-10 associates with polytene chromosomes in a general punctate pattern. The chromocenter is labeled (arrow), as are some euchromatic bands (arrowheads), and the tips of chromosomes 2L (B) and 4 (C), Bar in A, 15 μm. (B) Magnified view of area boxed in A showing that SU(VAR)2-10 (green) does not colocalize with HP1 (red) at the tip of chromosome 2L. (C) HP1 (red) densely stains the chromocenter and chromosome 4, and SU(VAR)2-10 (green) is found near the tip of chromosome 4. Bar, 10 μm. (D,E) SU(VAR)2-10 proteins localize distinctly to telomeres.