Figure 6.

SU(VAR)2-10 antibodies disrupt chromosome condensation and segregation in early embryos. Chromosome condensation and segregation are disrupted in embryos injected with anti-SU(VAR)2-10 antibodies (middle panels), but not in embryos injected with BSA (left panels) or α-amanitin (right panels), an RNA polymerase inhibitor. Embryos shown are at equivalent time points during the second mitotic cycle after injection. Time in minutes (t) postinjection is indicated for each panel. Note that at t = 25 metaphase chromosomes in the BSA- and α-amanitin-injected embryos are properly condensed and aligned at the metaphase plate. These nuclei divide and continue to cycle. During the second postinjection cycle in anti-SU(VAR)2-10-injected embryos, condensed metaphase chromosomes are never formed, and the mitotic cycles arrest prior to metaphase.