Table 3.
Experimental design for the three inducer system of Set II.
| Experimental conditions | E10 | E11 | E12 | E13 | E14 | E20 | E21 | E22 | E23 | E24 |
|---|---|---|---|---|---|---|---|---|---|---|
| Temperature (°C) | 60(0) | 45(−) | 45(−) | 75(+) | 75(+) | 60(0) | 75(+) | 45(−) | 75(+) | 45(−) |
| Time (h) | 18(0) | 12(−) | 24(+) | 12(−) | 24(+) | 18(0) | 24(+) | 12(−) | 12(−) | 24(+) |
| Ethanol concentration (%) | 60(0) | 45(−) | 75(+) | 75(+) | 45(−) | 60(0) | 75(+) | 75(+) | 45(−) | 45(−) |
| Antibacterial activity (cycle I) (Log CFU/mL) | 5.70 | 6.95 | 5.18 | 5.89 | 4.78 | 5.76 | 5.23 | 6.80 | 5.42 | 5.60 |
| Antibacterial activity (cycle II) (Log CFU/mL) | 5.55 | 7.08 | 5.38 | 5.69 | 5.00 | 5.57 | 5.40 | 6.99 | 5.28 | 5.39 |
| Difference (cycle I − cycle II) (Log CFU/mL) | 0.15 | −0.13 | −0.20 | 0.20 | 0.22 | 0.19 | −0.17 | −0.19 | 0.19 | 0.21 |
| Average activity (Log CFU/mL) | 5.625 (a10) | 7.015 (a11) | 5.280 (a12) | 5.790 (a13) | 4.890 (a14) | 5.665 (a20) | 5.315 (a21) | 6.895 (a22) | 5.350 (a23) | 5.495 (a24) |
Note: Numbers in parentheses are the coded symbols of levels of the extraction conditions.